Enhancers are genetic elements that regulate spatiotemporal gene expression. Enhancer function requires transcription factor (TF) binding and correlates with histone modifications (H3K27ac and H3K4me1). However, the extent to which TF binding and histone modifications can functionally define active enhancers remains unclear. We have combined chromatin immunoprecipitation with a massively parallel reporter assay to identify functional enhancers in human embryonic stem cells (hESCs) genome-wide in a quantitative unbiased manner. This website provides access to supplemental data for this resource.

Quick links

Enhancer search

Dynamic search tool for active enhancers in the proximity of genes of interest.

Name Description and link
Dynamic search tool
  • Search active enhancers in proximity of genes:
Full data download

Genome browser tracks

ChIP-seq, DNA-seq, and RNA-seq data for visualization as genome browser tracks. Related to Figure 1, Figure 4, and Figure 7 of the paper.

Name Description and link
Genome browser (GRCh37/hg19)
Selected tracks (GRCh37/hg19)
  • Selection of screenshots: PDF

Analysis results

Additional analysis results.

Name Description and link
Enhancer neighbourhood of primed/naive genes

Data and links

Links to raw data archives and related resources.

Name Description and link
Embryonic Stem Cell Biology
Medical Epigenomics
Related publications:
Software used for region overlap enrichment analysis used throughout our paper.


If you use this resource in your research, please cite:

Barakat TS*, Halbritter F*, Zhang M, Rendeiro AF, Bock C & Chambers I. (2018) Functional dissection of the enhancer repertoire in human embryonic stem cells. Cell Stem Cell,
doi: 10.1016/j.stem.2018.06.014